expression of microrna-155 is downregulated in peripheral blood mononuclear cells of chronic hepatitis b patients

Authors

su-lin yu department of infectious diseases, third affiliated hospital of sun yat-sen university, guangzhou, china

hong deng department of infectious diseases, third affiliated hospital of sun yat-sen university, guangzhou, china; department of infectious diseases, third affiliated hospital of sun yat-sen university, no. 600, tianhe road guangzhou, china. tel: +86-2085252063, fax: +86-2085252372

xin-hua li department of infectious diseases, third affiliated hospital of sun yat-sen university, guangzhou, china

ya-xin huang department of infectious diseases, third affiliated hospital of sun yat-sen university, guangzhou, china

abstract

conclusions the mir-155 appeared to be suppressed during hbv infection. the significantly higher mir-155 levels in alt-elevated patients infected with hbv suggest that mir-155 levels in pbmcs correlate with the immune state of patients with chronic hbv infection. results in the hbv-infected patients, the mir-155 levels were significantly lower than in the healthy controls (p = 0.001). chronic hbv-infected patients with elevated alt had higher levels of mir-155 compared with patients with normal alt (p = 0.014). no correlations were found between mir-155 and alt or hbv dna. patients and methods total rna was extracted from peripheral venous blood samples of 90 treatment-naive patients with chronic hbv infection and 20 healthy volunteers. the levels of mir-155 in the pbmcs were measured by real-time quantitative polymerase chain reaction. serum hbv dna and liver enzymes were estimated using standard clinical laboratory methods. background persistent hepatitis b virus (hbv) infection is sustained by inadequate immune responses, either natural or acquired. recent studies have suggested that immune responses to viral infection may be affected by microrna (mir)-155, via its involvement in immune cell differentiation and maturation. however, little is known on the specific interaction between mir-155 and hbv in host antiviral immunity. objectives this study evaluated the levels of mir-155 in peripheral blood mononuclear cells (pbmcs) of chronic hepatitis b (chb) patients, relative to that of healthy subjects, and investigated an association between mir-155 levels and hbv dna or alanine aminotransferase (alt).

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Expression of MicroRNA-155 is Downregulated in Peripheral Blood Mononuclear Cells of Chronic Hepatitis B Patients.

BACKGROUND Persistent hepatitis B virus (HBV) infection is sustained by inadequate immune responses, either natural or acquired. Recent studies have suggested that immune responses to viral infection may be affected by microRNA (miR)-155, via its involvement in immune cell differentiation and maturation. However, little is known on the specific interaction between miR-155 and HBV in host antivi...

full text

Negative Regulation of Semaphorin-3A Expression in Peripheral Blood Mononuclear Cells Using MicroRNA-497-5p

Background: Semaphorin-3A (Sema3A), as a secreted semaphorin, is an immune modulator molecule participating in the pathogenesis of autoimmune diseases. MicroRNAs (miRNAs) modulate the target-gene expression at the post-transcriptional level. It has been proposed that miRNAs may be crucial to the modulation of the function of semaphorins. Previous findings have proven that miR-497-5p is upregula...

full text

Mir-183 and FOXO1 gene expression changes in peripheral blood mononuclear cells of breast cancer patients

Background and Aim: Today, cancer is considered as a major health problem and affects the health of society. Breast cancer is the second leading cause of cancer death in women after lung cancer. According to epidemiological studies, cancer is the second most common cause of death after cardiovascular disease worldwide and the third leading cause of death after cardiovascular disease and acciden...

full text

Comparison of Hepatitis C Genotypes in Plasma and Peripheral Blood Mononuclear Cells (PMBCs) in Hepatitis C-Infected Patients

Abstract Background and Aims: The Hepatitis C Virus (HCV) is essentially hepatotropic but virus compartment has also been found in other important extra hepatic sites. Detection of HCV RNA in extra hepatic reservoirs such as peripheral blood mononuclear cells (PBMCs) is important for determining of disease progression and effective treatment. The present study aimed to determine of differe...

full text

Toll Like Receptor 2 and 4 Expression in Peripheral Blood Mononuclear Cells of Multiple Sclerosis Patients

Background: Multiple sclerosis (MS) is a T cell mediated autoimmune disease with unknown etiology. Appropriate MS therapeutic strategies need thorough understanding of both disease etiology and pathogenesis mechanisms. Ligation of TLR-2 and TLR-4 stimulates the production of several cytokines leading to CNS autoimmunity and neurodegenerative diseases. Objective: To find a relationship between M...

full text

Upregulated MicroRNA-155 Expression in Peripheral Blood Mononuclear Cells and Fibroblast-Like Synoviocytes in Rheumatoid Arthritis

OBJECTIVE This study was to screen for the miRNAs differently expressed in peripheral blood mononuclear cells (PBMC) of RA, to further identify the expression of miR-155 in RA PBMC and fibroblast-like synoviocytes (FLS), and to evaluate the function of miR-155 in RA-FLS. METHODS Microarray was used to screen for differentially expressed miRNAs in RA PBMC. miR-155 expression in PBMC and FLS of...

full text

My Resources

Save resource for easier access later


Journal title:
hepatitis monthly

جلد ۱۶، شماره ۱، صفحات ۰-۰

Keywords
[ ' h e p a t i t i s b ' , ' c h r o n i c ' , ' i m m u n i t y ' , ' a l a n i n e t r a n s a m i n a s e ' , ' l e u k o c y t e s ' , ' m o n o n u c l e a r ' , ' m i r ' , 1 5 5 ]

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023